The survey was carried out during 2013 – 2014, covering EljabelAlakther region in Libya. A total of 17 locations were included in this survey. To determine the frequency and abundance of plant-parasitic nematodes associated with Oleaeuropaeavaroleaster, a total of 68 composite soil samples were collected from the seventeen different areas in this region. Nematodes were extracted by sieving, Bearmanfunnel and root soaking, and then identified to the genus level. The results indicated that the common eight genera of plant-parasitic nematodes were identified as : Aphelenchus spp., Aphelenchiodes spp., Helicotylenchus spp., Pratylenchus spp. Paratylenchus spp., Tylenchorhynchus spp. Tylenchus spp., Xiphinemapachtaicum. Tylenchus spp. was recovered in 55% of the soil samples followed by Aphelenchusspp. 48%; the dagger nematode species was identified as X. pachtaicum using morphology and morphometrics of adult females.